Western blot analysis of cell lysates from A-498 cells treated for 0, 24, 48, and 72 hours with INFα (1,000 IU/ml) with antibodies against STAT1 (A) or known mediators of apoptosis including XIAP, FLIP, Bcl-2, Bcl-xL, Smac/DIABLO, Survivin, Caspase 8, and Caspase 3. For each of these experiments, staining for actin was done as a loading control (representative blots shown).