Table 4.
Surface tension and hydrophobicity (mJ · m−2) of the four pathogens determined from contact angle measurement.
Organism | pH | γ S LW | γ S + | γ S − | γ S AB | γ TOT | ΔG mwm 1 |
---|---|---|---|---|---|---|---|
A. hydrophila | 6 | 35.3 ± 1.0a | 1.9 ± 0.2b | 53.0 ± 0.4a | 20.0 ± 1.0b | 55.3 ± 0.1a | 26.3 ± 0.2b |
7 | 35.4 ± 0.7a | 1.5 ± 0.1b | 54.1 ± 0.3a | 18.2 ± 0.8b | 53.6 ± 0.1a | 28.7 ± 0.4a | |
8 | 35.8 ± 0.4a | 1.7 ± 0.0b | 53.6 ± 0.0a | 18.1 ± 0.0b | 53.9 ± 0.4a | 27.8 ± 0.3ab | |
| |||||||
E. coli 0157:H7 | 6 | 37.4 ± 0.1bc | 1.5 ± 0.2a | 52.3 ± 1.3a | 17.9 ± 1.3a | 55.3 ± 1.2a | 24.9 ± 0.8c |
7 | 38.5 ± 0.1a | 1.4 ± 0.1a | 54.0 ± 0.1a | 17.3 ± 0.3a | 55.8 ± 0.3a | 26.2 ± 0.0b | |
8 | 38.2 ± 0.6ab | 1.6 ± 0.2a | 53.5 ± 0.4a | 17.9 ± 1.0a | 56.0 ± 0.3a | 25,6 ± 0.4bc | |
| |||||||
S. enteritidis | 6 | 36.1 ± 0.7d | 1.7 ± 0.0a | 49.8 ± 0.8b | 18.3 ± 0.3a | 54.4 ± 0.5a | 23.0 ± 1.4a |
7 | 38.1 ± 0.0ab | 1.0 ± 0.1a | 51.1 ± 0.2ab | 14.0 ± 0.7a | 52.1 ± 0.7a | 25.1 ± 0.7a | |
8 | 38.6 ± 0.3a | 1.0 ± 0.2a | 52.0 ± 0.3a | 14.2 ± 1.3a | 52.8 ± 1.0a | 25.6 ± 0.9a | |
| |||||||
S. aureus | 6 | 37.5 ± 0.3b | 1.6 ± 0.1a | 46.0 ± 3.5b | 17.0 ± 0.3a | 54.5 ± 0.0a | 17.8 ± 4.4c |
7 | 39.7 ± 0.1a | 0.9 ± 0.2b | 54.6 ± 1.3a | 14.2 ± 1.1b | 53.8 ± 1.0a | 27.7 ± 2.1ab | |
8 | 36.5 ± 0.1b | 1.0 ± 0.1b | 54.7 ± 0.5a | 15.0 ± 0.4b | 51.6 ± 0.3b | 33.4 ± 0.8a |
1 Free energy of aggregation of the microorganisms in water where γ S LW, γ S +, γ S −, γ S B, and γ TOT are, respectively, the Lifshitz-van der Waals contribution energies, electron acceptor (acid), and electron donor (basic) components of the wetting agent and bacterial lawn, Lewis acid-base bonds and the total surface energy of the bacteria.
a–d In a column, for a given bacterium, values with the same letter are not significantly different (P > .05).