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. 2010 Sep 15;30(37):12545–12556. doi: 10.1523/JNEUROSCI.3042-10.2010

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Copyright © 2010 the authors 0270-6474/10/3012545-12$15.00/0

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Figure 6. — Inhibition of Rho kinase leads to accumulation of supporting cell phagosomes during aminoglycoside treatment. Wild-type chick utricles (E21) were incubated for 24 h in vitro with 1 mm streptomycin and 30 μm Y-27632. A, Phalloidin labeling reveals supporting cell phagosomes (arrows) within the epithelium of utricles treated with streptomycin alone. B, Simultaneous treatment with streptomycin and 30 μm Y-27632 increases the density of phagosomes within the epithelium. C, Y-27632 treatment significantly increases the number of supporting cell phagosomes observed (p < 0.001). Data from Figure 4 were used as controls for statistical tests in C. Data are expressed as mean ± SEM. Scale bars: 50 μm.

Inline graphic — Inhibition of Rho kinase leads to accumulation of supporting cell phagosomes during aminoglycoside treatment. Wild-type chick utricles (E21) were incubated for 24 h in vitro with 1 mm streptomycin and 30 μm Y-27632. A, Phalloidin labeling reveals supporting cell phagosomes (arrows) within the epithelium of utricles treated with streptomycin alone. B, Simultaneous treatment with streptomycin and 30 μm Y-27632 increases the density of phagosomes within the epithelium. C, Y-27632 treatment significantly increases the number of supporting cell phagosomes observed (p < 0.001). Data from Figure 4 were used as controls for statistical tests in C. Data are expressed as mean ± SEM. Scale bars: 50 μm.