Table 2. Michaelis-Menton parameters of wildtype and LRRK2 mutations for ATP.
The relative apparent Vmax (U/min) and Km (µM) for ATP were assessed with LRRKtide at 400 µM and Mg2+ or Mn2+ at 5 mM. The errors represent standard deviations. LRRK2, leucine-rich repeat kinase-2.
| Km |
Vmax |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mg |
Mn |
Mg |
Mn |
|||||||||
| Wildtype | 54.53 | +/− | 4.73 | 1.40 | +/− | 0.173 | 2.08 | +/− | 0.044 | 0.189 | +/− | 0.003 |
| R1441C | 62.97 | +/− | 4.02 | 2.61 | +/− | 0.472 | 3.06 | +/− | 0.049 | 0.238 | +/− | 0.006 |
| G2019S | 74.08 | +/− | 7.73 | 3.93 | +/− | 0.436 | 4.68 | +/− | 0.126 | 3.315 | +/− | 0.057 |
| I2020T | 18.13 | +/− | 2.63 | 0.95 | +/− | 0.153 | 2.67 | +/− | 0.078 | 0.179 | +/− | 0.004 |