Validation of DArT-identified
polymorphisms using genomic and subgenomic Southern blot technique.
Two clones (F4 and F8), representing the two polymorphic features
on the EcoRI Diversity panel were used as molecular
probes. Four varieties of rice were analysed simultaneously: lane
1, Bala; lane 2, Millin; lane 3, IR64; lane 4, IR20. (A)
Probe F4 and F8 hybridisation to the EcoRI genomic
Southern blots. (B) Probe F4 and F8 hybridisation
to the Southern blot of representations generated from genomic samples
analysed in (A).