Table 1. The adapter and primer oligonucleotide sequences.

Restriction enzyme	Adapter sequence	Primer sequence + selective bases at 3′ end of primer
	5′ 3′	5′3′
EcoRI	CTCGTAGACTGCGTACC	GACTGCGTACCAATTC-XXX	XXX = AAG, AGT, ACG, ATG
	AATTGGTACGCAGTCTAC
PstI	CACGATGGATCCAGTGCA	GATGGATCCAGTGCAG-X	X = T
	CTGGATCCATCGTGCAG
MspI	CGCATCGCAGTCTACA	GTAGACTGCGATGCGG-XX
	GACTGTAGACTGCGATG		XX= TG