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Low-temperature study (37 and 4 °C). Cells were preincubated at 37 °C (white bars) or 4 °C (gray bars) for 1 h and further incubated in the same conditions with PNA705 conjugates of RXR4, RXR6, RXR8, RXR8−4B1, or RXR8−2B, peptides conjugates at 1 μM. Luciferase expression assays were carried out after 24 h as indicated in the Experimental Procedures. Experiments were made in triplicate, and error bars are indicated. Data are expressed as the percentage of luciferase activity.
