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Kpna7 gene mutation caused loss of KPNA7 protein expression in the mouse. A–D, KPNA7-specific antibody staining in wild type GV (A), MII (B) oocytes, zygotes (Zy, C), and two-cell embryos (D). E–H, KPNA7-specific antibody staining in Kpna7 mutation GV (E), MII (F) oocytes, zygotes (G), and two-cell embryos (H). Significant and specific positive signal had been observed in oocytes and early embryos that isolated from wild type mice but not Kpna7 mutation mice (bars, 20 μm).
