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. 2010 Aug 10;285(43):33113–33122. doi: 10.1074/jbc.M110.117044

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — Biochemical analysis of the interaction between KPNA7 and KPNB1 and proposed models. A and B, mouse KPNA7 and KPNB1 interaction analysis. Western blotting analysis of samples obtained by immunoprecipitation. Expression vectors were cotransfected into 293T cells, respectively. Kb1 and Ka7 represent KPNB1 and KPNA7, respectively. A, KPNB1 protein (Kb1-FG) is used as the bait protein and FLAG antibody is used to perform immunoprecipitation. KPNA2 (Ka2-his) is used as a positive control. KPNA7 protein with deleted IBB domain is used as a negative control. B, KPNA7 and KPNB1 are used as bait proteins, respectively, to further confirm the interaction between KPNA7 and KPNB1. C, proposed model in cell level for how the KPNA7 mutation caused the phenotypes/abnormalities.