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. 2010 Aug 20;285(43):32744–32750. doi: 10.1074/jbc.M110.137588

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — GPIs of T. gondii associate with galectin-1 through their lipid moiety. SPR analysis of the interaction of immobilized galectin-1 with (A) the six different T. gondii GPIs (I-VI) at 3 μm; (B) GPI III at different concentrations (Scatchard plot shown in the inset, slope = −1.77); (C) GPIa at different concentrations; (D) diacylglycerols isolated from GPI III at different concentrations; or (E) laminin at 10 μg/ml. Results are expressed as RU (proportional to the quantity of molecules) after the subtraction of the nonspecific binding. Sensorgrams are representative of independent experiments with similar results.