Skip to main content
. 2010 Aug 11;285(43):32787–32792. doi: 10.1074/jbc.M110.151340

FIGURE 4.

FIGURE 4.

MyD88 is target gene of NF-κB. A, MyD88 promoter-luciferase activity followed by Western blot analysis for MyD88 expression in Raw cells treated with LPS in the presence of parthenolide. B, MyD88 promoter-luciferase activity in Raw cells treated with LPS after cotransfecting with p65. C, four NF-κB motifs in mouse MyD88 promoter. The NF-κB consensus sequences were identified by using the program alibaba 2.1. The functionally active NF-κB sites are in bold. D, ChIP analysis of p65, p65 followed by RNA polymerase II, and MTA1 followed by p65 recruitment to MyD88 chromatin in Raw cells treated with LPS for 1 h. E, effect of parthenolide on the recruitment of p65 onto MyD88 promoter (+81 to −93 and −280 to −569) in LPS-stimulated Raw cells. F, EMSA analysis of p65 binding to the mouse MyD88 promoter using the oligonucleotide encompassing NF-κB consensus sequences (Site 1, Site 2, and Site 3) in Raw cells treated LPS for 2 h.