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Aurora-A activation of AR depends on phosphorylation of Thr282 and Ser393. A and B, reporter assay. PC3 (A) and HEK293 (B) cells were transfected with the indicated plasmids, treated with and without R1881. After 36 h of incubation, cells were assayed for luciferase activity. The bottom panels of A–D show the expression of transfected plasmids. All of the experiments were repeated three times in triplicate. *, p < 0.05. Error bars, S.E.
