FIGURE 6.

Knockdown of Aurora-A in LNCaP-RF cells reduces PSA expression and androgen-independent phenotype; Aurora-A-phosphorylated AR induces cell growth in the presence and absence of androgen. A, knockdown of Aurora-A decreases PSA expression. LNCaP-RF cells were transfected with shRNA-Aurora-A and scramble shRNA and detected for expression of Aurora-A (top two panels) and PSA (panels 3 and 5) as described in the legend to Fig. 5. Actin was used as a control. B and C, knockdown of Aurora-A reduces LNCaP-RF androgen-independent phenotype. Aurora-A/shRNA- and scramble shRNA-treated LNCaP-RF cells were cultured in phenol red-free RPMI 1640 medium containing 10% charcoal-stripped serum and then assayed for cell growth (B) and apoptosis (C). D and E, AR-2D but not AR-2A induces cell proliferation in the absence and presence of R1881. PC3 (D) and LNCaP (E) cells were seeded in a 6-well plate (10⁵ cells/well) and then transfected with the indicated plasmids (bottom panels). Following incubation for 4 days in phenol red-free RPMI 1640 medium containing 10% charcoal-stripped serum in the absence or the presence of R1881, cell number was counted. The experiments were repeated three times in triplicate. *, p < 0.05. Error bars, S.E.