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. 2010 Aug 11;285(43):32937–32945. doi: 10.1074/jbc.M110.144014

FIGURE 5.

FIGURE 5.

The amounts of Tenebrio PGRP-SA and 17-kDa protein in the hemolymph increased after injection of polymeric Lys-type and DAP-type PGs. A and B represent Western blot analysis using anti-PGRP-SA and anti-PGRP-SC2 antibodies, respectively. Lanes 1 and 2 indicate the purified PGRP-SA (500 ng) and the purified PGRP-SC2 (500 ng), respectively. Lanes 3-5, lanes 6–8, and lanes 9–11 represent the injection of insect saline (IS), monomeric DAP-type PG (mDAP), and polymeric DAP-type PG (pDAP) after 12 h, 1 days (1d), and 2 days (2d), respectively. At the indicated times, hemolymph was collected, and then a portion (40 μg of protein) of each sample was analyzed by immunoblotting using affinity-purified anti-Tenebrio PGRP-SA and PGRP-SC2 antibodies. Ab, antibody.