TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Reference or source |
|---|---|---|
| E. coli strains | ||
| W3110 | Wild type, F−, λ− | E. coli Genetic Stock Center (Yale) |
| XL-1 Blue | recA1 endA1 gyrA96thi-1 hsdR17 supE44 relA1 lac (F′ proAB lacIqZΔM15::Tn10 (TetR)) | Stratagene |
| Rosetta(DE3)pLysS | F−ompT hsdSB(rB− mB−) gal dcm (DE3) pLysSRARE (CamR) | Novagen |
| TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL StrrendA1 nupG | Invitrogen |
| CWG904 | TOP10 carrying the plasmid, pWQ480; Apr | This work |
| CWG905 | Rosetta(DE3)pLysS carrying the plasmid, pWQ481; Kmr | This work |
| CWG906 | TOP10 carrying the plasmid, pWQ482; Apr | This work |
| CWG907 | TOP10 carrying the plasmid, pWQ483; Apr | This work |
| CWG908 | TOP10 carrying the plasmid, pWQ484; Apr | This work |
| CWG909 | TOP10 carrying the plasmid, pWQ485; Apr | This work |
| Plasmids | ||
| pBAD24 | l-Arabinose-inducible plasmid; Apr | (25) |
| pET28a | Cloning/expression vector; Kmr | Novagen |
| pWQ480 | pBAD24 derivative encoding LptC-His6; Apr | This work |
| pWQ481 | pET28a derivative encoding His6-LptC24–191; Kmr | This work |
| pWQ482 | pBAD24 derivative encoding LptC24–191-TEV-His6; Apr | This work |
| pWQ483 | pBAD24 derivative encoding LptC-His6 with insertion of cysteine (C2); Apr | This work |
| pWQ484 | pBAD24 derivative encoding LptC-His6 with insertion of cysteine (C186); Apr | This work |
| pWQ485 | pBAD24 derivative encoding LptA-TEV-His6; Apr | This work |