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. 2010 Aug 18;285(43):33529–33539. doi: 10.1074/jbc.M110.144709

TABLE 2.

Oligonucleotides used for cloning

Primers are 5′ → 3′.

Name	Sequence	Description
MT5	`GCGCGCGAATTCACCATGAGTAAAGCCAGACGT`	FP^a: LptC-His₆
MT6	`GCGCGCTCTAGATCAGTGGTGGTGGTGGTGGTGAGGCTGAGTTTGTTTGTT`	RP: LptC-His₆
MT30	`GAGCTACATATGGCCGAAAAAGACGATACC`	FP: His₆-LptC_24–191
MT27	`GATGCTGGATCCTTAAGGCTGAGTTTGTTT`	RP: His₆-LptC_24–191
MT28	`CAGGAGGAATTCACCATGAATGATCCCACCTATAAAAGC`	FP: LptC_24–191-TEV-His₆
MT56	`GCGCGCTCTAGACTAATGATGATGATGATGATGACCCTGAAAATACAGGTTTTCACCGGTACCAGGCTGAGTTTGTTTGTTTTG`	RP: LptC_24–191-TEV-His₆
MT37	`CAGGAGGAATTCACCATGTGTAGTAAAGCCAGACGTTGGG`	FP: LptC-His₆ (C2)
MT38	`CCCAACGTCTGGCTTTACTACACATGGTGAATTCCTCCTG`	RP: LptC-His₆ (C2)
MT39	`GAACATCCTATGAAATTCAATGTAACAAACAAACTCAGCC`	FP: LptC-His₆ (C186)
MT40	`GGCTGAGTTTGTTTGTTACATTGAATTTCATAGGATGTTC`	RP: LptC-His₆ (C186)
MT1	`GCGCGCGAATTCACCATGAAATTCAAAACAAAC`	FP: LptA-TEV-His₆
MT55	`GCGCGCTCTAGACTAATGATGATGATGATGATGACCCTGAAAATACAGGTTTTCACCGGTACCATTACCCTTCTTCTGTGCCGG`	RP: LptA-TEV-His₆

^a FP, forward primer; RP, reverse primer.