FIGURE 4.
Modulation of the catalytic activity of SIRT1. Closed symbols correspond to activation of the deacetylation of desTAMRA-peptide. Data points were fit to Equation 1 and yielded: 22, EC50 = 1.7 ± 0.1 μm, RVmax = 2.6 ± 0.1; 23, EC50 = 2.2 ± 0.3 μm, RVmax = 2.8 ± 0.2; 24, EC50 = 1.5 ± 0.1 μm, RVmax = 2.1 ± 0.1. Open symbols correspond to inhibition of the deacetylation p53 20-mer. Data points were fit to the equation RV = 1/(1+([X]o/Ki, apparent)) and yielded: 22, Ki, apparent = 9 ± 4 μm; 23, Ki, apparent = 10 ± 1 μm; 24, Ki, apparent = 9 ± 3 μm.