Enhanced lipid anabolism in EDL muscle of MPGC-1α TG animals. A–D, FAS gene expression, activity, de novo lipogenesis, and accumulation of intramyofibrillar lipids, respectively, in glycolytic muscle of MPGC-1α TG versus wild-type animals. E, relative expression of genes involved in fatty acid uptake (lipoprotein lipase (LPL), CD36, plasma membrane fatty acid-binding protein (FABPpm), and fatty acid transporter proteins (FATPs)), fatty acid activation (ACS), and esterification (acetyl-CoA:diacylglycerol acyltransferase (DGAT) and mitochondrial glycerol-3-phosphate acyltransferase (mtGPAT)). All values are expressed as means ± S.E. (n = 6–8/group). *, p < 0.05; **, p < 0.01; ***, p < 0.001.