FIGURE 4.

Caveolin-1 expression enhances the formation of lipid droplets. Cells were incubated with OlAc for 48 h and labeled with anti-FLAG antibodies. A, the addition of OlAc to the culture medium causes lipid storage droplets in caveolin-1-expressing cells and redistribution of caveolin-1 (red in merged pictures) to ring-shaped cytoplasmic structures identified as lipid droplets by BODIPY 493/503 (green in merged pictures). In contrast, the mutant did not form lipid droplets. B, an enlarged image of the section bounded by the white box in A to illustrate the location of the FLAG tag surrounding the lipid droplet.