FIGURE 2.
UV-C and UV-B irradiation induces eIF2∼P in different cell types. Human glioblastoma cells M059K (A) and normal human keratinocytes (NHK) cells (B) were treated with 40 J/m2 UV-C irradiation and then cultured for up to 6 h. As a control, cells were exposed to 1 μm TG for 6 h. Lysates were prepared from the stressed human cells, and the levels of the indicated proteins were measured by immunoblot analysis. Wild-type MEF cells (C) and normal human keratinocyte cells (D) were treated with increasing dosages of UV-B irradiation and cultured for up to 6 h, and the indicated proteins were measured by immunoblot analyses. Each panel is representative of three independent experiments.