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. 2010 Aug 19;285(43):32824–32833. doi: 10.1074/jbc.M110.121087

FIGURE 2.

FIGURE 2.

A, effects of p38 inhibitor SB203580 (1, 5, and 10 μm) on SRP72 protein expression was evaluated by WB using antibodies against human SRP72, SRP54, and GAPDH. A decreased intensity of SRP72 bands was noted when using the inhibitor at 5 μm concentration at 240 min (lane 6) and 10 μm at 120 and 240 min (lanes 8 and 9). B, results were analyzed and RUA illustrated, finding significant results at 5 μm, 240 versus 0 min, and 10 μm, 240 versus 0 and 120 versus 0 min, respectively, (p < 0.05). C, Jurkat cells with SB202190 at 1, 5, and 10 μm were tested, and a decreased SRP72 expression was found when using at 10 μm (lanes 8 and 9). D, results were analyzed and RUA illustrated, finding significant results at 10 μm at 240 versus 0 and 120 versus 0 min (p < 0.05). E, JNK inhibitor SP600125 (1, 5, and 10 μm, 0, 120, and 240 min); no difference in intensity of bands corresponding to SRP72, SRP54, and GAPDH was found. F, RUA of JNK inhibitor by WB was illustrated, without significant changes in protein expression of SRP72, SRP54, and GAPDH. The experiments were repeated three times.