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. 2010 Jul 30;285(43):33404–33412. doi: 10.1074/jbc.M110.114058

FIGURE 5.

FIGURE 5.

Co-expression of mutant β3 but not β3-WT destabilizes the fast component of inactivation. A, all β3-subunits increased the proportion of channels inactivating with a slower time constant relative to Nav1.3/no β3 cells. The increase was significant for Nav1.3/β3-WT and Nav1.3/β3-ΔSE, but Nav1.3/β3-AP was not significantly different from Nav1.3/no β3 or Nav1.3/β3-WT. Sample numbers for the fast and slower components were reduced at more positive conditioning potentials where test pulse current amplitudes were too small to fit. B, shown is a comparison of the voltage sensitivity of the fast gating channels. Means (±S.E.) were generated from the following sample numbers: Nav1.3/no β3, n = 20; Nav1.3/β3-WT, n = 20; Nav1.3/β3-AP, n = 15; Nav1.3/β3-ΔSE, n = 13. Conditioning potentials where the amplitude of the fast component was significantly greater (p < 0.05) for the mutant β3-subunits relative to Nav1.3/β3-WT were −60 mV (Nav1.3/β3-AP and Nav1.3/β3-ΔSE) and −75 mV (Nav1.3/β3-ΔSE).