TABLE 1.

Time to peak and kinetics of inactivation for Na_v1.3 expressed alone or with β3-subunits

Co-expression of β3-WT with Na_v1.3 increases the time to reach peak current and slows the rate of inactivation using a family of 20-ms depolarizations (“Experimental Procedures”). All β3-subunits significantly increased the mean time to peak, increased the proportion of channels that decay with a slower time constant, reduced the proportion of channels that decay with the fast time constant, and increased the time constant for the slow component of current decay (τ_slow). Means (±S.E.) were generated from the sample numbers shown (n). Significant differences from Na_v1.3/no β3 are indicated in the footnotes.

β3-Subunit	Time to peak at peak I	Time to peak at V½	Current decay					n
			Amplitude of slow	τSlow	Amplitude of fast	τFast	Amplitude of persistent
	ms	ms	%	ms	%	ms	%
No β3	0.61 ± 0.01	0.98 ± 0.03	16.08 ± 1.51	4.49 ± 0.43	79.35 ± 1.94	0.70 ± 0.02	4.57 ± 0.60	27
β3-WT	0.82 ± 0.03a	1.25 ± 0.05a	36.80 ± 4.01a	7.65 ± 0.48a	56.58 ± 4.62a	0.75 ± 0.04	6.62 ± 0.78	24
β3-AP	0.85 ± 0.05a	1.34 ± 0.06a	36.64 ± 6.29b	7.22 ± 0.68c	54.35 ± 7.51b	0.76 ± 0.06	9.00 ± 1.86	14
β3-ΔSE	0.81 ± 0.03a	1.34 ± 0.07a	38.05 ± 3.88b	8.44 ± 1.21a	52.39 ± 5.83b	0.84 ± 0.11	9.56 ± 2.93	12

^a p < 0.001.

^b p < 0.01.

^c p < 0.05.