FIGURE 3.

Loss of daily rhythms of Srebp-1 expression in Clock mutant mice under the conditions of ad libitum feeding (A) and fasting (B). Male Clock mutant (mutation-homozygous) mice were housed in a 12:12LD with feeding ad libitum for at least 2 weeks. For fasting, mice were food-depleted at ZT9 (Day 0). Total RNA was prepared from the liver of mice at 4-h intervals on Day 2, and subjected to Northern analysis for measuring Srebp-1 mRNA levels, as described in the legend of Fig. 1. The broken line represents the wild-type 12:12LD/ad libitum results reproduced from Fig. 1A, for comparison.