FIGURE 7.

Effects of varied nutrient compositions on Srebp-1 expression. Male wild-type C57BL/6 mice were fed ad libitum with a standard chow in 12:12LD for at least 2 weeks, and then fed with various chows enriched in carbohydrate (A), fat (B), and protein (C) for 7 days. Daily rhythms of hepatic Srebp-1 (top panels), Dbp (second panels from the top) and Rev-erbα (third panels from the top) mRNA levels were examined by Northern analysis, and those of SREBP-1 protein levels (bottom panels) by Western analysis, as described in the legend of Fig. 1. The broken lines for comparison represent the results reproduced from Fig. 1.