FIGURE 5.

Transgenic expression of dn HIF1α in adipose tissue reduces energy expenditure, lipid utilization, and thermogenesis in mice. Indirect calorimetry was performed by housing 28-week-old aP2/dn HIF1α transgenic mice and wild type littermates under a high fat diet in a six-chamber Oxymax Lab Animal Monitoring System. A, VO₂ during a 24-h light and dark cycle. B, oxygen consumption expressed as area under curve. C, RER (VCO₂/VO₂) during the 24-h light and dark cycle. D, RER expressed as area under curve. E, average core body temperature measured during 24-h light and dark cycle. F, changes in core body temperature when exposed to cold temperature. *, p < 0.05 versus WT littermates by Student's t test (n = 5–6). Error bars, S.E.