FIGURE 7.

Increased adipocyte sizes and elevated inflammation in adipose tissue of 32-week-old aP2/dn Hif1α transgenic mice on standard chow. A, representative microscopic images from histological sections of epididymal fat pads stained with hematoxylin and eosin. SC, standard chow; HF, high fat diet. Scale bar, 10 μm. B, real time PCR analysis to quantify the mRNA expression levels for several proinflammatory markers in epididymal fat pads under high fat diet. The relative abundance of each gene was normalized against 18 S RNA and expressed as -fold over wild type littermates (n = 5–9). *, p < 0.05; **, p < 0.01 versus WT controls. C, typical microscopic photos from histological sections of BAT. Scale bar, 10 μm. Error bars, S.E.