FIGURE 2.

Stress-induced keratin 8/18/20 phosphorylation is p38-dependent. A, HT29 cells were stimulated with 1 or 10 μg/ml anisomycin (Aniso) for 30 min. For p38 inhibition, cells were pretreated with 5 μm SB203580/5 μm SB202190 for 1 h followed by a 30-min stimulation with 10 μg/ml anisomycin. After treatment, the samples were analyzed by Western blotting using antibodies to the indicated antigens. GAPDH was used as a loading control. B, HT29 cells grown on coverslips were left untreated or stimulated for 30 min with anisomycin, with or without pretreatment with 5 μm SB202190. Cells were fixed and stained using antibodies to the indicated antigens.