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. 2010 Aug 19;285(43):33242–33251. doi: 10.1074/jbc.M110.132357

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — MK2 directly phosphorylates K18 and K20 in vitro. A, comparison of phosphorylation sites in K18/20 with that of MK2 substrates mHSPB1/hHSPB1 and consensus MK2/3 phosphorylation site. Matching residues are in bold, and the phosphorylated S/T is denoted. B, in vitro kinase assay with GST-p38/His-MK2 followed by Western blotting for K8-Ser⁷³ phosphorylation and for total K8 protein. C, in vitro kinase assay for K20-Ser¹³ phosphorylation. mHSPB1-Ser⁸⁶ and MK2-Thr³³⁴ phosphorylations are shown as controls. D, in vitro kinase assay for K18-Ser⁵² phosphorylation.