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Keratin 20 phosphorylation is abrogated in MK2/3−/− mice ileum. A, ileum tissue from three WT and MK2/3-double deficient mice each were processed as mentioned under “Materials and Methods” and subjected to Western blotting using the indicated antibodies. B, small intestinal villi from WT and MK2/3−/− mice stained for K20-Ser13 (blue) and nuclei (white) show reduced levels of phosphorylation at the base of villi (see insets).
