Skip to main content
. 2010 Aug 11;285(43):33092–33103. doi: 10.1074/jbc.M110.130377

FIGURE 5.

FIGURE 5.

C/EBPβ binding activity to the CCL3(−300/−141) and CCL4(−222/−101) promoter regions is increased in the presence of IL-1β. A chromatin immunoprecipitation assay was performed using anti-C/EBPβ antibodies in T/C-28 cells treated with IL-1β for various times as indicated. Treatment with IL-1β enhanced the binding of C/EBPβ to the promoter. A, T/C-28 cells were treated with 1 ng/ml IL-1β for 0, 1, 4, 8, and 24 h. B, T/C-28 cells were treated with or without IL-1β (1 ng/ml) for 1 h. C and D, T/C-28 cells were treated with 1 ng/ml IL-1β for 0 and 24 h, and the relative expression levels of input and C/EBPβ antibody DNAs of CCL3 (C) and CCL4 (D) were examined by the quantitative real time PCR method. The value was normalized to GAPDH, and the expression levels of input and C/EBPβ at 0 h were set as 1. E, T/C-28 cells were treated with 1 ng/ml IL-1β for 0, 4, 8, and 24 h, and the relative expression levels of CCL3 of input and C/EBPβ antibody DNAs were examined by the quantitative real-time PCR method. The value was normalized to GAPDH and input, and the expression levels of C/EBPβ at 0 h were set as 1. Error bars, S.D.