FIGURE 7.

Silencing of C/EBPβ suppresses the IL-1β-induced CCL3 and CCL4 transcriptional activation. A, Odyssey Western blot of C/EBPβ proteins in T/C-28 cells transfected with control (Ctrl) or C/EBPβ siRNAs (162 pm), and stimulated with 1 ng/ml IL-1β for 8 h. B, T/C-28 cells were transfected with CCL3 and CCL4 promoters and, where indicated, cotransfected with control (ctrl) or C/EBPβ siRNAs (162 pm). Twenty-four h after transfection, T/C-28 cells were stimulated with 1 ng/ml IL-1β for 24 h (CCL3) or 8 h (CCL4). Relative luciferase activity indicates the -fold expression relative to the activity of promoter with IL-1β (1 ng/ml) only (set as 1). C, T/C-28 cells transfected with control or C/EBPβ siRNAs were stimulated for 8 h with IL-1β (1 ng/ml). The relative expression levels were examined by the quantitative real time PCR method. The p value of C/EBPβ siRNA was compared with IL-1β alone based on Student's t test (**, p < 0.01). Each bar represents the mean ± S.D. (error bars) of at least three independent experiments.