FIGURE 9.

Transcriptional regulation in the highly expression of CCL3 and CCL4. A, the candidate C/EBPβ and NF-κB binding sites in human CCL3 (−1395) and CCL4 (−1281) constructs. B, C/EBPβ and IKK2 stimulate the expression of CCL3 (−1395) and CCL4 (−1281) promoter in T/C-28 human chondrocytes. The CCL3 (−1395) and CCL4 (−1281) promoter constructs were co-transfected with C/EBPβ and IKK2 expression plasmid into T/C-28a2 cells with or without IL-1β (1 ng/ml) for 24 h (CCL3) or 8 h (CCL4). Relative luciferase activity indicates the -fold expression relative to the activity of the construct co-transfected with empty vector (set as 1) in the presence of IL-1β after each co-transfection compared with their activities in the absence of IL-1β. Each bar represents the mean ± S.D. (error bars) of three independent experiments.