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. 2010 Aug 9;285(43):32919–32926. doi: 10.1074/jbc.M109.062513

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — 5-HT influences Lbc subcellular localization. A, serum-starved PASMC were stimulated with 1 μm 5-HT, then fractionated into cytosolic (S) or membrane-rich (P) fractions, and immunoblotted for 107-kDa Lbc, cytoplasmic dynein (cytosolic control), and PDGF receptor (PDGFR) for membrane control. A representative experiment of three is shown; the graph quantitates the S:P ratio of the relative densitometric values of Lbc in each fraction from three experiments. ∧, p < 0.05 for 5-HT-treated versus untreated cells. Bars = S.D. B, PASMC transfected with FLAG:proto-Lbc plasmid were serum starved, then stimulated with 1 μm 5-HT and stained with FLAG antibody followed by Alexa-conjugated secondary antibody for Lbc detection by confocal microscopy; the z-slice was 0.33 μm. Arrows indicate peripheral Lbc localization in 5-HT-treated cells.