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. 2010 Aug 9;285(43):32919–32926. doi: 10.1074/jbc.M109.062513

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Attenuation of 5-HT-induced mitogenesis and SRE. L-mediated reporter activation in PASMC by Lbc knockdown. A, Lbc immunoblot of PASMC transfected with 25 nm scr or Lbc (L1) siRNA after 48 h. B and C, PASMC were either mock-transfected (control and 5-HT) or transfected with scr or L1 siRNA (12 and 25 nm) (B) or with 25 nm LARG GEF siRNA (LG) and L1 siRNA + human Lbc (hLbc) plasmid (C). After 48 h serum withdrawal, cells were treated with 5-HT overnight and assayed for [³H]thymidine uptake. n = 6, ∧, p < 0.05 for 5-HT + scr groups versus control; *, p < 0.05 for 5-HT + L1 versus 5-HT + scr groups; #, p < 0.05 for 5-HT + L1 + hLbc plasmid versus 5-HT + L1. D, PASMC transfected with scr or L1 siRNA were serum withdrawn, then treated with PDGF overnight and assayed for [³H]thymidine uptake. ∧∧, p < 0.05 for PDGF + scr versus scr. E, PASMC transfected with SRE.L luciferase reporter + siRNAs were serum-starved for 48 h, then treated with 5-HT for 5 h, and luciferase levels were measured. RLU, relative luciferase units. ∧, p < 0.05 for 5-HT group versus untreated control (reporter); *, p < 0.05 for L1 versus scr group, n = 3. Bars = S.D.