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. Author manuscript; available in PMC: 2010 Oct 25.
Published in final edited form as: Cytometry B Clin Cytom. 2010;78(Suppl 1):S98–109. doi: 10.1002/cyto.b.20544

Fig. 5.

Fig. 5

CFU-pre-B cell assay on sorted SP and non-SP. A, Flow cytometry cell sorting scheme for spleen cells which were 7AAD− and gated on IgM− and sorted into SP and non-SP, and then further into SP B220− population (17-month-old NZB shown as an example). B, Sorted cells were grown in Methocult pre-B cell media for 7 days and cells were harvested. Representative flow cytometric contour plots for NZB SP cells (top rows) and C57Bl/6 SP cells (bottom rows) harvested from 7 days Methocult culture. Percentage of cells in each quadrant is indicated.