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. Author manuscript; available in PMC: 2010 Oct 25.
Published in final edited form as: Gastroenterology. 1984 Jun;86(6):1410–1416.

Figure 5.

Figure 5

Gel filtration chromatography of estrogen binding proteins from male rat liver and Morris hepatoma 7777 cytosol. Cytosol (4 ml) from normal liver (open circles) was chromatographed on Sephadex G-100 and estrogen binders were detected as indicated in Methods. Hepatoma (open circles) cytosol was incubated for 2 h with 2 nM [3H]E2 before chromatography on Sephadex G-100. A 400-μl aliquot of each fraction was assayed for [3H]E2 content. Markers used to calibrate the column included blue dextran (Vo); bovine serum albumin (B), 68.000; ovalbumin (O), 43,000; trypsinogen (T), 24,000; myoglobin (M), 17,000.