FIG. 4.

The effect of differential dietary treatment on the regulation of metabolic fluxes in the liver. AICAR-stimulated fatty acid oxidation (A) and insulin-stimulated de novo fatty acid synthesis (B) in cultured hepatocytes isolated from wild-type and AMPKα2^−/− mice fed for 9 weeks either a Chow diet, cHF, or cHF+F. For basal nonstimulated rates of lipid metabolism, see supplementary Table 3. The expression of SCD-1 (C) and SREBP-1c (D) genes was quantified in total RNA isolated from the livers of mice subjected to hyperinsulinemic-euglycemic clamp following the differential dietary treatment for 9 weeks. The data are means ± SE (isolated hepatocytes, n = 3 in triplets; hepatic gene expression, n = 5–8). *P < 0.05 versus genotype Chow; †P < 0.05 versus genotype cHF; ‡P < 0.05 versus wild-type on respective diet. A.U., arbitrary units.