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Circulating levels of FGF21 are increased in mice with diet-induced obesity. Analysis of FGF21 mRNA expression in diet-induced obese mice is shown. mRNA expression was quantified using a standard quantitative PCR technique. Data are shown from the liver (A) and WAT (B). C: Circulating FGF21 levels were determined with the use of an ELISA in serum from a terminal bleed. Data are displayed as the mean ± SE. ***P < 0.001.
