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. 2010 Aug 3;59(11):2781–2789. doi: 10.2337/db10-0193

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© 2010 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 3. — Induction of immediate early gene expression downstream of FGF21 signaling is impaired in obese mice. To confirm whether FGF21 signaling is impaired in obese mice, we assessed immediate early gene expression downstream of ERK in obese and lean mice. Both mRNA and protein levels were analyzed in the liver (A) and WAT (B). For each tissue, mRNA fold increase is displayed as a bar graph and a Western blot showing Egr1 protein expression presented below. cFos mRNA is also shown in the liver (C) and WAT (D). Data are means ± SE.