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. 2010 Aug 16;59(11):2960–2971. doi: 10.2337/db10-0367

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© 2010 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 5. — Immune cell repertoire in adipose tissue of 6-week-old (6 weeks) or 6-month-old (6 months) B6 and 129 mice. Cells in the stromavascular fraction were labeled with fluorescence-conjugated antibodies against different myeloid cell and lymphoid markers and counted using flow cytometry. Nonerythrocytes stromavascular cells, PI-/Ter-119-; leukocytes, PI-/Ter-119-/CD45+; macrophages, PI-/Ter-119-/CD45+/CD11b+/F4/80+/CD11c-; CD11c⁺-Myeloid cells, PI-/Ter-119-/CD45+/CD11b+/F4/80+/CD11c+; T-cells, PI-/Ter-119-/CD45+/CD3+; T-helper cells, PI-/Ter-119-/CD45+/CD3+/CD4+. A: Represents number of cells per gram of fat tissue and (B) the total number of cells in the epididymal fat depot. All values are mean ± SEM of 4–8 animals. *P < 0.05; **P < 0.01; ***P < 0.001 vs. 129 mice. #P < 0.05; ##P < 0.01; ###P < 0.001 vs. 6 weeks.