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. Author manuscript; available in PMC: 2010 Oct 25.
Published in final edited form as: J Hepatol. 1989 Jan;8(1):32–41. doi: 10.1016/0168-8278(89)90159-1

Fig. 1.

Fig. 1

Effect of cimetidine, ranitidine, famotidine and omeprazole on DNA synthesis of hepatocytes in primary culture. Hepatocyte isolation has been described under ‘Materials and Methods’. Cells were plated in MEM supplemented with 5% FCS and insulin (10−7 M). After 3 h, the medium and floating cells were discarded and serum-free medium plus insulin (10−7 M), EGF (10 ng/ml) and proline (0.26 mM) were added. H2 blocking agents were added at different concentrations on the basis of their blood level after administration of a therapeutic dose; cimetidine, ranitidine, famotidine (0.62–30 μg/ml) and omeprazole (0.06–1.8 μg/ml). Cultures were exposed to 7.5 μCi [3H] thymidine for the last 24 h and processed after 48 h for determination of DNA synthesis. For each medium, triplicate culture dishes from 5 different animals were used. Results (mean ± S.D.) are expressed as cpm × 10−2/μg DNA.