TABLE 1. Effect of Cimetidine, Ranitidine, Famotidine and Omeprazole on Viability, DNA Concentration and Transaminase Levels of Hepatocytes in ‘primary cultures’.
Hepatocyte isolation has been described under ‘Materials and Methods’. Cells were plated in MEM supplemented with 5% FCS and insulin (10−7 M). After 3 h attachment, the medium and floating cells were removed and serum-free MEM plus insulin (10−7 M) was added. Antisecretory agents were added as indicated. Cells were cultured for 72 h and processed every 24 h for viability and DNA determinations. ALT was determined and expressed as U/ml only for the dishes stopped after 72 h of incubation. For each time point, triplicate culture dishes were assayed. The results are expressed as the average from 9 dishes and 3 different animals (mean ± S.D.).
| 24 h | 48 h | 72 h | ALT (U/ml) | ||||
|---|---|---|---|---|---|---|---|
| Viability (%) | DNA (μg/dish) | Viability (%) | DNA (μg/dish) | Viability (%) | DNA (μg/dish) | ||
| Control | 95 ± 3 | 3.2 ± 0.2 | 97 ± 3 | 3.3 ± 0.2 | 98 ± 2 | 3.28 ± 0.3 | 9± 3 |
| Cimetidine 27 × 1C−6 g/ml | 97 ± 2 | 3.1 ± 0.2 | 98 ± 2 | 3.2 ± 0.3 | 98 ± 1 | 3.28 ± 0.2 | 11 ± 5 |
| Ranitidine 6.0 × l0−6g/ml | 98 ± 1 | 3.1 ± 0.3 | 97 ± 3 | 3.4 ± 0.35 | 96 ± 4 | 3.05 ± 0.4 | 10 ± 9 |
| Famotidine 2.1 × l0−6g/ml | 95 ± 2 | 3.2 ± 0.1 | 96 ± 1 | 2.95 ± 1.2 | 97 ± 1 | 3.08 ± 0.7 | 12 ± 3 |
| Omeprazole 1.8 × l0−6g/ml | 95 ± 1 | 3.2 ± 0.1 | 97 ± 2 | 2.9 ± 1.1 | 97 ± 2 | 3.3 ± 0.9 | 8± 2 |