TABLE 2. DNA Concentration and Labelling Index of Hepatocyte Cultures Grown in the Presence of Cimetidine, Ranitidine, Famotidine, and Omeprazole.
Culture conditions were as for Fig. 1. After 48 h of incubation, dishes were processed for DNA concentration and % labelled nuclei. Concentrations of H2 blocking agents were 30 times higher than the blood value found in patients injected with one pharmacological dose of the drugs. Cultures without H2 blocking agents were used as control. DNA concentration was expressed as μg/dish. The results are expressed as the average from 9 dishes and 3 different animals (mean ± S.D.). DNA in the labelled nuclei of hepatocytes incubated in the presence of insulin alone was 2.8 ± 0.33 μg/dish; percent of labelled nuclei in the same conditions was 4.5 ± 7.
| DNA (μg/dish) | Labelled nuclei (%) | |
|---|---|---|
| Control (saline) | 6.2 ± 1.2 | 10 ± 1 |
| Cimetidine 27 × 10−6g/ml | 5.2 ± 1.8 | 9± 2 |
| Ranitidine 6.0 × 10-6g/ml | 5.8 ± 1.2 | 10 ± 2 |
| Famotidine 2.1 × 10-6g/ml | 5.5 ± 2.0 | 12 ± 2 |
| Omeprazole 1.8 × 10-6g/ml | 5.9 ± 1.8 | 11 ± 3 |