Fig. 1.

Ten14 stimulates PLCγ1 preferentially over ERK/MAPK. Quiesced NR6WT cells were stimulated with indicated doses of EGF or Ten14 for 5 min at 37°C. Cells were then lysed, proteins separated through SDS–PAGE gels, and immunoblotted for the indicated proteins. Shown are representative blots of at least four for each analysis. A: Both Ten14 and EGF simulate PLCγ1 to equivalent levels at comparable concentrations of ligand. However, ERK/MAPK is weakly phosphorylated even at the high concentrations of Ten14. B: PLCγ1 and SHC are robustly activated even by nanomolar levels of Ten14. However, unlike EGF, the phosphorylation of ERK/MAPK and p90RSK remains weak even at micromolar concentrations of Ten14.