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. Author manuscript; available in PMC: 2010 Oct 25.
Published in final edited form as: Helicobacter. 2006 Oct;11(5):477–493. doi: 10.1111/j.1523-5378.2006.00441.x

Figure 1.

Figure 1

Development of an H. pylori chromosomal complementation system. (A) Wild-type 26695 H. pylori chromosome region chosen for complementation. The intergenic region (IR) was divided into two fragments for cloning purposes, IR203 and IR204. Arrow indicates direction of transcription for the coding regions. The 326 bp IR203 and 332 bp IR204 regions were cloned. The actual intergenic region size in strain 26695 is shorter (632 bp), based on sequence analysis (see Fig. 6 and Table 4). (B) Construction of cloning vectors: pIR20304, pIR203C04, pMLB001, pMLB002, pMLB003, and pMLB004. Arrows in plasmids denote direction of transcription. MCS, multicloning site.