Table 2. Primers and Restriction Endonucleases used for genotyping IL-15 gene.
SNP ID | Primer sequences | Product | Annealing | Restriction |
Length (bp) | temperature(°C) | Endonucleases | ||
rs10519612 | F: 5′-CTCAATGTCCTTAACCCATTATTCGA-3′ | 137 | 56.1 | BspT104 I |
R: 5′-CGTTTGAACCATATGGTGAGGTCT-3′ | ||||
rs10519613 | F: 5′-TAGACATAACAAAACACTCGGCATTT-3′ | 122 | 59.3 | Dra I |
R: 5′-CTCAATGACATTTTTCTGCCTTCA-3′ | ||||
rs35964658 | F: 5′-GTTCTGAAACTTTTCATCAAATGAGC-3′ | 149 | 62.4 | Hha I |
R: 5′-CTAGCCTTACTGGGGAAAATGG-3′ | ||||
rs17007695 | F: 5′-GTCTTTCTCATGGTCCTCATTGA-3′ | 126 | 56.1 | BspT104 I |
R: 5′-AAAATTTACAAATGTGTGATTTTCGA-3′ | ||||
rs17015014 | F: 5′-CGGACTGCTGGGTCTAAGAAGCTA-3′ | 119 | 62.4 | Xsp I |
R: 5′-GTCTGACTCATCAGCCAACACCC-3′ |
F = Forward Primer; R = Reverse Primer.