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. 2010 Oct 25;5(10):e13626. doi: 10.1371/journal.pone.0013626

Table 2. Primers and Restriction Endonucleases used for genotyping IL-15 gene.

SNP ID Primer sequences Product Annealing Restriction
Length (bp) temperature(°C) Endonucleases
rs10519612 F: 5′-CTCAATGTCCTTAACCCATTATTCGA-3′ 137 56.1 BspT104 I
R: 5′-CGTTTGAACCATATGGTGAGGTCT-3′
rs10519613 F: 5′-TAGACATAACAAAACACTCGGCATTT-3′ 122 59.3 Dra I
R: 5′-CTCAATGACATTTTTCTGCCTTCA-3′
rs35964658 F: 5′-GTTCTGAAACTTTTCATCAAATGAGC-3′ 149 62.4 Hha I
R: 5′-CTAGCCTTACTGGGGAAAATGG-3′
rs17007695 F: 5′-GTCTTTCTCATGGTCCTCATTGA-3′ 126 56.1 BspT104 I
R: 5′-AAAATTTACAAATGTGTGATTTTCGA-3′
rs17015014 F: 5′-CGGACTGCTGGGTCTAAGAAGCTA-3′ 119 62.4 Xsp I
R: 5′-GTCTGACTCATCAGCCAACACCC-3′

F = Forward Primer; R = Reverse Primer.