Table 3. Wnt pathway gene expression in diabetes-exposed embryos assayed by Affymetrix microarray (430 2.0).

Gene expression levels for selected known Wnt pathway genes in a comparison between diabetes-exposed and control embryos at day 10.5 of gestation. Details are published elsewhere (Pavlinkova et al., submitted). Signals for expression of Wnt3a, probes for which are represented on the 430 assay) were below the limit of detection in all samples. It is noteworthy that all components of the Wnt signaling pathway exhibit decreased expression in diabetes-exposed embryos (D) compared to controls (N), as reflected in the negative fold-change values.

Gene symbol	Affymetrix probe ID	P value (CyberT)	Fold change D vs. N	Gene name
Apc	1450056_at	0.022	−3.6	adenomatosis polyposis coli
Ctnnb1	1430533_a_at	0.039	−12.9	catenin (cadherin associated protein), beta 1
Dixdc1	1444395_at	0.009	−7.3	DIX domain containing 1
Dvl2	1417207_at	0.002	−2.5	dishevelled 2, dsh homolog (Drosophila)
Frzb	1448424_at	0.013	−3.0	frizzled-related protein
Fzd1	1422985_at	0.039	−7.2	frizzled homolog 1 (Drosophila)
Fzd2	1418532_at	0.012	−2.7	frizzled homolog 2 (Drosophila)
Fzd4	1449416_at	0.045	−11.3	frizzled homolog 4 (Drosophila)
Fzd7	1450044_at	0.015	−2.2	frizzled homolog 7 (Drosophila)
Gsk3b	1451020_at	0.039	−2.3	glycogen synthase kinase 3 beta
Sfrp1	1416594_at	0.002	−5.1	secreted frizzled-related protein 1
Wnt5a	1448818_at	0.042	−2.1	wingless-related MMTV integration site 5A
Wnt7b	1420892_at	0.0066	−2.5	wingless-related MMTV integration site 7B