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. 2010 Sep;30(9):677–681. doi: 10.1089/jir.2009.0117

FIG. 4.

FIG. 4.

Treatment with the PI3 kinase inhibitor LY294002 or the p38 MAPK inhibitor SB203580 decreased both the phosphorylation of CREB and TGFβ2 expression in Z12 cells. (A, B) Western analyses, showing that phosphorylated CREB decreased following treatment with LY294002 (40 μM) or SB203580 (20 μM). Z12 cells were treated for 24 h and analyzed by the Western method with an antibody to phosphorylated CREB. (C, D) Northern analyses, showing that the level of TGFβ2 mRNA in Z12 cells was decreased following treatment with LY294002 (40 μM) or SB203580 (20 μM). Total RNAs (10 μg) were analyzed by the Northern method, with a TGFβ2 probe. CREB, cyclic adenosine monophosphate (cAMP)-responsive element binding protein; TGFβ2, transforming growth factor β2.