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. Author manuscript; available in PMC: 2010 Oct 25.
Published in final edited form as: J Cell Physiol. 2005 Jul;204(1):210–218. doi: 10.1002/jcp.20285

Fig. 5.

Fig. 5

Nuclear and cytoplasmic fractionation of HA-Abs and Myc-SNX2. CHO were transfected with a combination of HA-Abs, Myc-SNX2, and empty pcDNA3.1(+) vector control and fractionated using the NE-PER kit. One quarter of each fractions were analyzed by Western immunoblot with anti-HA and anti-Myc antibodies. Histone deacetylase-1 (HDAC-1) and GDP-dissociation inhibitor (GDI)α were used as nuclear and cytosolic controls, respectively. Lanes indicated with C represents the cytosolic fraction and N the nuclear fraction.